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Alexey FILIPPOV, Maria KUZNETSOVA, Alexander ROGOZHIN, Olga IAKUSHEVA, Valentina DEMIDOVA, Natalia STATSYUK
《农业科学与工程前沿(英文)》 2018年 第5卷 第4期 页码 462-468 doi: 10.15302/J-FASE-2018239
Late blight is one of the most important potato diseases. To minimize yield losses, various protective measures are used including fungicide application. Active use of fungicides results in a contamination of the environment. Therefore, crop protection strategies optimizing the number of treatments are of great interest. Using information about late blight development in an experimental potato field recorded over 30 seasons, a simulator to forecast yield losses caused by the disease was developed based on the number of 5-d periods favorable for reinfection of plants during a vegetation season. The simulator was successfully verified using independent data on the disease development from nine unprotected potato fields in the Netherlands and Germany. The average difference between the calculated and real yield losses did not exceed 5%. Using the simulator and weather data for a period of 2007-2017, yield losses were calculated for several areas of the Bryansk, Tambov, and Orenburg Regions of Russia. The results revealed differences in disease development between these regions and may be used to develop recommendations for a frequency of fungicide applications according to the regional risk of epidemics, leading to a significant reduction in fungicide use.
关键词: potato late blight Phytophthora infestans yield losses retrospective analysis mathematical model
Identification of molecular markers linked to rice bacterial blight resistance genes from
Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN
《农业科学与工程前沿(英文)》 2015年 第2卷 第3期 页码 260-265 doi: 10.15302/J-FASE-2015070
关键词: Oryza meyeriana bacterial blight resistance gene genetic population linkage molecular marker
《农业科学与工程前沿(英文)》 2021年 第8卷 第2期
Late embryogenesis abundant (LEA) genes encode highly hydrophilic proteins that are essential in abiotic stress responses. However, most LEA genes in higher plants have not yet been investigated. This study identified an LEA family gene (PtrLEA7) from Poncirus trifoliata and studied its function in drought tolerance. The full-length coding sequence of PtrLEA7 was 420 bp encoding a protein of 139 amino acids. Phylogenetic analysis shows that PtrLEA7 protein belongs to the LEA_4 subfamily. Expression profiling by qPCR found that PtrLEA7 was strongly induced by dehydration, cold and ABA treatments, and slightly induced by salt stress. Subcellular localization reveals that PtrLEA7 protein was located in both cytoplasm and nucleus. To investigate its function, transgenic plants of both tobacco and Poncirus trifoliata overexpressing PtrLEA7 were obtained. Stress tolerance assays show that overexpression lines had enhanced dehydration and drought tolerance compared with wild type plants, indicating that PtrLEA7 positively regulates drought tolerance. In addition, transgenic plants had much higher expression levels of three antioxidant enzyme genes (CAT, SOD and POD) and significantly increased catalase enzyme activity, accompanied by reduced reactive oxygen species accumulation in comparison with wild type plants. Collectively, this study demonstrates that PtrLEA7 can confer enhanced drought tolerance partially via enhancing antioxidant capacity.
Breeding for the resistance to
Hongxiang MA, Xu ZHANG, Jinbao YAO, Shunhe CHENG
《农业科学与工程前沿(英文)》 2019年 第6卷 第3期 页码 251-264 doi: 10.15302/J-FASE-2019262
With the changes of climate and cultivation systems, the head blight (FHB) epidemic area in China has extended since 2000 from the reaches of the Yangtze River to the north and west winter wheat region. Breeding for FHB resistance in wheat is an effective way to control the disease. Chinese wheat breeders commenced research on FHB in the 1950s. Sumai 3, Ning 7840, Yangmai 158, Ningmai 9 and other cultivars with improved FHB resistance were developed through standard breeding methods and widely applied in production or breeding programs. In addition to intervarietal crosses, alien germplasm was used to improve FHB resistance of wheat. Addition, substitution and translocation lines with alien chromosomes or chromosome fragments were created to enhance FHB resistance. Somaclonal variation was also used to develop a FHB resistant cv. Shengxuan 3 and other cultivars with moderate resistance to FHB were released by such methods. QTL (quantitative trait loci) for FHB resistance were characterized in cultivars originating from China. The major QTL, , was identified on chromosome 3BS in Sumai 3, Ning 894037, Wangshuibai and other Chinese resistant sources. Diagnostic molecular markers for 1 have been applied in wheat breeding and breeding lines with improved FHB resistance and desirable agronomic traits have been obtained. However, breeding for FHB resistance is a long-term task, new technologies are likely to increase the efficiency of this process and better FHB resistance of new cultivars is expected to be achieved within the next decade.
Tonglu WEI, Dalong GUO, Jihong LIU
《农业科学与工程前沿(英文)》 页码 236-246 doi: 10.15302/J-FASE-2020368
关键词: abiotic stress antioxidant drought late embryogenesis abundant Poncirus trifoliata
胡东生,胡蓉,李小豫,胡文瑄,王旭龙,田新红,刘卫国,张国伟
《中国工程科学》 2012年 第14卷 第11期 页码 36-43
通过庐山科学考察(1997—2009)对大校场沟口晚第四纪剖面地层进行精细研究,采用古地磁、电子自旋共振(ESR)、36Cl、释光法等多种仪器测年方法的分析,建立了完整剖面的全时段地层的自400 ka.B.P.以来的系列年龄数据,与之相对应识别出Biwa-III(320 ka.B.P.)事件、Biwa-II(260 ka.B.P.)事件、Biwa-I(180 ka.B.P.)事件(因铁盘扰动影响缺失)、Blake(100 ka.B.P.)事件、Laschamp(20 ka.B.P.)事件(因表层活动影响未发育)等古地磁极性事件,结合层序地层和层型特征及沉积环境将剖面地层由早至晚划分为4个发展旋回阶段:I阶段为冰期(400~200 ka.B.P.)气候时期,II阶段为间冰期(200~100 ka.B.P.)气候时期,III阶段为冰缘期(100~10 ka.B.P.)气候时期,IV阶段为冰后期(10~0 ka.B.P.)气候时期。
Jihong LU, Danfeng ZHANG, Qian CHEN, Buwei YU
《化学科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 19-25 doi: 10.1007/s11705-010-0546-1
关键词: late transition metal catalyst methyl methacrylate polymerization salicylaldiminato nickel complexes methylaluminoxane syndiotactic structure
初生多肽相关复合物调控小麦禾谷镰刀菌生长发育和致病性 Article
王旭丽, 谢鑫, 刘敬, 王国梁, 邱德文
《工程(英文)》 2020年 第6卷 第5期 页码 546-552 doi: 10.1016/j.eng.2019.07.025
对植物病原菌而言,维持蛋白质内稳态对其生存和繁殖至关重要。然而,当前对参与维持蛋白质内稳态的因子在真菌致病过程中的作用仍缺乏科学的认识。在本研究中,我们鉴定了十大病原真菌之一—禾谷镰刀菌(Fusarium graminearum)的初生多肽相关复合物(FgNAC)的功能。研究发现,FgNAC中的α亚基(FgNACα)与其在酵母和其他物种中的同源基因在结构和功能方面表现出高度相似性。禾谷镰刀菌NACα缺失突变体虽可存活但其菌丝生长、分生孢子产生和致病性受到严重削弱。此外,通过酵母双杂交实验发现FgNACα可以和FgNACβ亚基发生互作。亚细胞定位结果表明FgNACα和FgNACβ主要发生在细胞质中。未来的研究应致力于解析初生多肽相关复合物调节蛋白质代谢从而调控真菌发育和致病性的作用机制。
Maura Massimino, Marta Podda, Lorenza Gandola, Emanuele Pignoli, Ettore Seregni, Carlo Morosi, Filippo Spreafico, Andrea Ferrari, Emilia Pecori, Monica Terenziani
《医学前沿(英文)》 2021年 第15卷 第1期 页码 101-107 doi: 10.1007/s11684-020-0752-2
关键词: iatrogenic primary hypothyroidism late effects of radiotherapy long-term follow-up medulloblastoma Hodgkin lymphoma
标题 作者 时间 类型 操作
Development and testing of a weather-based model to determine potential yield losses caused by potato lateblight and optimize fungicide application
Alexey FILIPPOV, Maria KUZNETSOVA, Alexander ROGOZHIN, Olga IAKUSHEVA, Valentina DEMIDOVA, Natalia STATSYUK
期刊论文
Identification of molecular markers linked to rice bacterial blight resistance genes from
Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN
期刊论文
OVEREXPRESSION OF PTRLEA7, A LATE EMBRYOGENESIS ABUNDANT FAMILY GENE FROM PONCIRUS TRIFOLIATA, CONFERS
期刊论文
Polymerization of methyl methacrylate catalyzed by mono-/bis-salicylaldiminato nickel(II) complexes and methylaluminoxane
Jihong LU, Danfeng ZHANG, Qian CHEN, Buwei YU
期刊论文
Long-term results of suppressing thyroid-stimulating hormone during radiotherapy to prevent primary hypothyroidism in medulloblastoma/PNET and Hodgkin lymphoma: a prospective cohort study
Maura Massimino, Marta Podda, Lorenza Gandola, Emanuele Pignoli, Ettore Seregni, Carlo Morosi, Filippo Spreafico, Andrea Ferrari, Emilia Pecori, Monica Terenziani
期刊论文